Primer Design
With this primer design tool, provided by Integrated DNA Technologies, the user can analyze the input sequence (up to 255 bases) obtaining helpful data: GC content, molecular weight, complementary sequence, a representative image of the hairpin, self-dimer and heterodimer structures formation of the primer sequences… Moreover, Oligo Analyzer 3.1, allows you to Blast your primer …
Calculate the Tm (Melting Temperature, in Celsius degrees) of your short primers (less than 14 bases) following this formula: Tm = 4(G+C) + 2(A+T)
Calculate the Tm (Melting Temperature, in Celsius degrees) of your primers: For long primers use the formula : Tm = 64.9 + 41(((G+C) -16.4)/N) Where N is the primer length in bases. This formula can also be used for shorter primers and is more accurate for long primers than the short primer formula.
Primer 3
Primer-BLAST
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