The MicroRNAs (miRNAs) can be defined as small, non-coding RNA. This miRNAs usually have a about 20 to 22 nucleotides. The main known role of this miRNAs is that they negatively regulate gene expression at post-transcriptional level. miRNAs play important roles in different pathological and physiological processes. Taken this into account, the identification and analysis of differentially expressed miRNAs across tissues/cell lines becomes quite a hot area of study due to the gene regulation process driven by this miRNAs.
With mirTools 2.0 the user can identify the targets of microRNAs, functional annotate targets genes and evaluate the ncRNAs profiling. This free online tool provides an intuitive interface:
- To start the analysis, first input the reads file in fasta or SAM/BAM mapped format.
- Choose the options for analysis (check the help tab if you have any questions about the analysis).
- To perform the analysis Click ‘submit’.
- Retrieve the result of analysis.
To check the help documentation for mirTools 2.0 -visit here.