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Jak/Stat: IL-6 receptor family

JAK stands for Janus kinase. This is a family of intracellular, nonreceptor tyrosine kinases that transduce cytokine-mediated signals via the JAK-STAT pathway. The JAK-STAT signaling pathway transmits information from chemical signals, through STAT activation, into gene promoters on the DNA activating the DNA transcription.  

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Cell cycle/Checkpoint

Cell cycle control: G1/S checkpoint. G2/M damage checkpoint.

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Translational control

Translational control: mTOR. Translational control overview. Regulation of elF4E and p70 S6K. Regulation of elF2.

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Apoptosis signaling

Apoptosis signaling pathways: Regulation of Apoptosis Overview. Inhibition of Apoptosis. Death receptor signaling. Mitochondrial control of apoptosis.

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Chromatin regulation

Chormatin regualtion: Histone Lysine Methylation. Protein Acetylation. Examples of crosstalk between post-translational modifications.    

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Cell Staining Simulation Tool

With Cell Staining Simulation Tool developed by Life Technologies, you can compare different dyes and areas of staining within the cells. To create the perfectly fluorescent labelled cell the user only needs to choose the cellular area to stain, and select the fluorescent dye color. After your selection, you will find out the best combination […]

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Research Methods

Internet provides a broad number online resources that could help you on a daily basis research. Here I enclose a few websites that I found really useful in my lab work. Enjoy the protocols and improve your techniques following the advices of experts in the subject. Would you like to share your protocol with the […]

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Bacterial cell number (OD600)

Based on the reading of the spectrophotometer at Optical Density of 600nm, you can calculate the concentration of bacteria following this formula:    

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Histone methylation

Histones have been shown to be dynamic proteins, undergoing multiple types of post-translational modifications. In this pathway presented by Cell signaling you will find a brief description of the main histone modifications, both in the transcriptionally active and inactive chromatin.

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Simple Interactive Statistical Analysis

After the procedure selection and fill in the form by the user, Simple Interactive Statistical Analysis (SISA) analyzes statistically the provided data. SISA also provides a friendly user guide to helps in the statistical test choice.

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