Another great online tool from New England Biolabs INC. NEB Tm Calculator is used to estimate an appropriate annealing temperature in NEB PCR products. The tool fields are: Product Group: select a value from Q5, Q5 Hot Start, Q5U Hot Start, OneTaq, OneTaq Hot Start, Hot Start Taq, Taq DNA Polymerase, LongAmp Taq, LongAmp Hot …
JAK stands for Janus kinase. This is a family of intracellular, nonreceptor tyrosine kinases that transduce cytokine-mediated signals via the JAK-STAT pathway. The JAK-STAT signaling pathway transmits information from chemical signals, through STAT activation, into gene promoters on the DNA activating the DNA transcription.
Cell cycle control: G1/S checkpoint. G2/M damage checkpoint.
Translational control: mTOR. Translational control overview. Regulation of elF4E and p70 S6K. Regulation of elF2.
Apoptosis signaling pathways: Regulation of Apoptosis Overview. Inhibition of Apoptosis. Death receptor signaling. Mitochondrial control of apoptosis.
Chormatin regualtion: Histone Lysine Methylation. Protein Acetylation. Examples of crosstalk between post-translational modifications.
With Cell Staining Simulation Tool developed by Life Technologies, you can compare different dyes and areas of staining within the cells. To create the perfectly fluorescent labelled cell the user only needs to choose the cellular area to stain, and select the fluorescent dye color. After your selection, you will find out the best combination …
Internet provides a broad number online resources that could help you on a daily basis research. Here I enclose a few websites that I found really useful in my lab work. Enjoy the protocols and improve your techniques following the advices of experts in the subject. Would you like to share your protocol with the …
Based on the reading of the spectrophotometer at Optical Density of 600nm, you can calculate the concentration of bacteria following this formula:
Histones have been shown to be dynamic proteins, undergoing multiple types of post-translational modifications. In this pathway presented by Cell signaling you will find a brief description of the main histone modifications, both in the transcriptionally active and inactive chromatin.