Cloning

Vector Builder is a really useful free online tool where you can design (and purchase) a custom-built vector with a few clicks. In three steps you could design your expression vector, or order targeting vectors and Bac modification vectors. Selecting your requirements in Vector Builder you can construct your vector and the map will be …

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Plas Mapper, a free plasmid drawing website, automatically generates plasmid maps providing the DNA sequenze (up to 20 Kb) in FASTA format.

This online tool, provided by Promega, will allow you to calculate the molar ratio insert to vector, taking into account the lengh of the vector and insert (in kb) and the amount of vector (in ng).