To determine the stable housekeeping genes in your experiment you can use this excel-based tool using pair-wise correlations. The first step in your qPCR gene expression analysis shoul be the test of your housekeeping (or reference) genes that usually are assumed that present a stable expression among samples, but could be affected by some …
Phylodendrom is a phylogenetic tree printer, once you have the newick or nh format of your data, you can upload the file to this site and select the phylogenetic tree style that you want to represent (cladogram, phenogram, eurogram…). Find a brief Phylodendrom manual here.
With this online tool you can calculate the % of efficiency of the PCR reaction, the exponent and the amplicication from the slope produced by the quantitative PCR standard curve. Slope values between -3.1 and -3.6 are acceptable, and they will give reaction efficiencies between 90% and 110%. The formula to calculate the efficiency is …
Based on the reading of the spectrophotometer at Optical Density of 600nm, you can calculate the concentration of bacteria following this formula:
Calculate the nucleic acid concentration of your DNA, RNA or oligonucleotides samples at the Optical Density 260 nm (OD260). Different concentrations of DNA, RNA and oligos have different ability aborbing light. At OD260, the Absorbance of light and the nucleic acid concentration is calculated as follows: where: C: the concentration of the nucleic acid, in …
Calculate the Tm (Melting Temperature, in Celsius degrees) of your short primers (less than 14 bases) following this formula: Tm = 4(G+C) + 2(A+T)
Calculate the Tm (Melting Temperature, in Celsius degrees) of your primers: For long primers use the formula : Tm = 64.9 + 41(((G+C) -16.4)/N) Where N is the primer length in bases. This formula can also be used for shorter primers and is more accurate for long primers than the short primer formula.
Histones have been shown to be dynamic proteins, undergoing multiple types of post-translational modifications. In this pathway presented by Cell signaling you will find a brief description of the main histone modifications, both in the transcriptionally active and inactive chromatin.
Many microcentrifuges only have settings for speed (revolutions per minute, RPM), not relative centrifugal force, but to be more accurate, certain procedures necessitate precise centrifugation conditions, which must be specified in terms of relative centrifugal force (RCF) expressed in units of gravity (times gravity or × g). The relationship between revolutions per minute (RPM) and …
MEGA (Molecular Evolutionary Genetics Analysis). MEGA is really useful tool for conducting sequence alignment, testing evolutionary hypotheses, inferring phylogenetic trees, estimating rates of molecular evolution, inferring ancestral sequences and mining web-based databases. In the website, MEGA offers useful tools likean Introductory book containing many examples for use with MEGA, a step-by-step instructions to learn how …
