Use NEBcutter2.0 tool to find the restriction sites within your DNA sequence, identifiying the sites for both Type II and comercially available Type III restriction enzymes. Enter your DNA sequence (maximun length 300KBases) and click on “submit” to find the restriction sites. Visit the original link: https://nc3.neb.com/NEBcutter/ The original tool is not working? visit the …
Basic Logical Alignment Search Tool BLAST (Basic Local Alignment Search Tool) is a bioinformatics tool that is used to search for similar sequences in a database. It compares a query sequence against a database of sequences and finds the highest scoring matches. English Tutorial BLAST (Basic Local Alignment Search Tool) is a bioinformatics tool that …
Another great online tool from New England Biolabs INC. NEB Tm Calculator is used to estimate an appropriate annealing temperature in NEB PCR products. The tool fields are: Product Group: select a value from Q5, Q5 Hot Start, Q5U Hot Start, OneTaq, OneTaq Hot Start, Hot Start Taq, Taq DNA Polymerase, LongAmp Taq, LongAmp Hot …
Laboratory quality control is designed to detect deficiencies in the analytical process, in order to reduce, correct and improve the quality of the analysis and results developed in the lab. The aim of the quality control is to evaluate the results reproducibility, that is a measure of precision to know how well the laboratory technique …
JAK stands for Janus kinase. This is a family of intracellular, nonreceptor tyrosine kinases that transduce cytokine-mediated signals via the JAK-STAT pathway. The JAK-STAT signaling pathway transmits information from chemical signals, through STAT activation, into gene promoters on the DNA activating the DNA transcription.
Cell cycle control: G1/S checkpoint. G2/M damage checkpoint.
Translational control: mTOR. Translational control overview. Regulation of elF4E and p70 S6K. Regulation of elF2.
Apoptosis signaling pathways: Regulation of Apoptosis Overview. Inhibition of Apoptosis. Death receptor signaling. Mitochondrial control of apoptosis.
Chormatin regualtion: Histone Lysine Methylation. Protein Acetylation. Examples of crosstalk between post-translational modifications.
The MicroRNAs (miRNAs) can be defined as small, non-coding RNA. This miRNAs usually have a about 20 to 22 nucleotides. The main known role of this miRNAs is that they negatively regulate gene expression at post-transcriptional level. miRNAs play important roles in different pathological and physiological processes. Taken this into account, the identification and analysis …
