Calculate the Tm (Melting Temperature, in Celsius degrees) of your short primers (less than 14 bases) following this formula:
Tm = 4(G+C) + 2(A+T)
Calculate the Tm (Melting Temperature, in Celsius degrees) of your short primers (less than 14 bases) following this formula:
Tm = 4(G+C) + 2(A+T)
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