-
Codon Table (Standard Genetic Code)
The codon table (standard genetic code) maps each of the 64 three-base codons to an amino acid or a stop signal. Use […]
-
GC Content Calculator
Calculate the GC content of a DNA or RNA sequence — the percentage of guanine and cytosine bases. Paste your sequence to […]
-
DNA and RNA Quantification: Concentration, Purity and Yield
How to measure the concentration, purity and yield of DNA and RNA — the spectrophotometric and fluorometric methods, what the ratios mean, […]
-
qPCR Data Analysis: Efficiency, Reference Genes and ΔΔCq
This guide walks through analysing qPCR data: building a standard curve, checking amplification efficiency, choosing reference genes, and quantifying with the ΔΔCq […]
-
Restriction Enzyme Cloning: A Step-by-Step Guide
Restriction–ligation cloning is still the workhorse for moving a fragment into a vector. This guide covers the whole workflow — choosing enzymes, […]
-
How to Design PCR Primers: A Step-by-Step Guide
Good primers are the difference between a clean PCR band and a smear of non-specific products. This guide walks through the rules […]
-
NEBcutter V3.0
NEBcutter V3.0 is New England Biolabs’ tool for finding the restriction enzyme sites in a DNA sequence. Paste your sequence into the […]
-
Blast NCBI+ Tutorials (EN/ES)
BLAST (Basic Local Alignment Search Tool) finds regions of similarity between your sequence and the sequences in NCBI’s databases. Run a search […]
-
mirTools 2.0
The MicroRNAs (miRNAs) can be defined as small, non-coding RNA. This miRNAs usually have a about 20 to 22 nucleotides. The main […]
-
Oligo Analyzer
Analyze the key properties of a DNA oligonucleotide instantly: length, GC content, melting temperature (Tm) and molecular weight. Paste your oligo below […]