Molecular Genetics
With this online tool you can calculate the % of efficiency of the PCR reaction, the exponent and the amplicication from the slope produced by the quantitative PCR standard curve. Slope values between -3.1 and -3.6 are acceptable, and they will give reaction efficiencies between 90% and 110%. The formula to calculate the efficiency is …
Calculate the nucleic acid concentration of your DNA, RNA or oligonucleotides samples at the Optical Density 260 nm (OD260). Different concentrations of DNA, RNA and oligos have different ability aborbing light. At OD260, the Absorbance of light and the nucleic acid concentration is calculated as follows: where: C: the concentration of the nucleic acid, in …
Calculate the Tm (Melting Temperature, in Celsius degrees) of your short primers (less than 14 bases) following this formula: Tm = 4(G+C) + 2(A+T)
Calculate the Tm (Melting Temperature, in Celsius degrees) of your primers: For long primers use the formula : Tm = 64.9 + 41(((G+C) -16.4)/N) Where N is the primer length in bases. This formula can also be used for shorter primers and is more accurate for long primers than the short primer formula.
Plas Mapper, a free plasmid drawing website, automatically generates plasmid maps providing the DNA sequenze (up to 20 Kb) in FASTA format.
This online tool, provided by Promega, will allow you to calculate the molar ratio insert to vector, taking into account the lengh of the vector and insert (in kb) and the amount of vector (in ng).
RE Database
RestrictionMapper
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