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How to Design PCR Primers: A Step-by-Step Guide
Good primers are the difference between a clean PCR band and a smear of non-specific products. This guide walks through the rules […]
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Oligo Analyzer
Analyze the key properties of a DNA oligonucleotide instantly: length, GC content, melting temperature (Tm) and molecular weight. Paste your oligo below […]
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Tm of PCR primers (<14 bp)
Estimate the melting temperature (Tm) of short PCR primers and oligonucleotides (roughly ≤ 14 bp). Paste your primer below: the calculator shows the quick […]
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Tm of long PCR primers
Calculate the Tm (Melting Temperature, in Celsius degrees) of your primers: For long primers use the formula : Tm = 64.9 + […]
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Primer 3
Primer 3
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Primer-BLAST
Primer-BLAST