Primer 3
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Oligo AnalyzerAnalyze the key properties of a DNA oligonucleotide instantly: length, GC contenTm of long PCR primersCalculate the Tm (Melting Temperature, in Celsius degrees) of your primers: For How to Design PCR Primers: A Step-by-Step GuideGood primers are the difference between a clean PCR band and a smear of non-spec