SearchLight is a free fluorescence spectra and filter-set viewer from Semrock (IDEX Health & Science), used to design and check filter sets for fluorescence microscopy and flow cytometry — matching fluorophores to excitation/emission filters and dichroics.
What you use it for
- Overlay fluorophore spectra with filter and dichroic transmission curves.
- Estimate signal and bleed-through for a given filter set.
- Compare and design multi-channel configurations to minimise crosstalk.
Related
For a broader look at fluorophore selection and spectral overlap (including flow cytometry), see our fluorescence spectra viewer guide, which links the BD, Thermo Fisher and FPbase viewers.
Frequently asked questions
What is Semrock SearchLight?
A free online tool for overlaying fluorophore spectra with optical filter and dichroic curves to design fluorescence filter sets.
How do I choose a filter set?
Match the excitation filter to the dye’s excitation peak and the emission filter to its emission, while minimising overlap between channels — SearchLight visualises this.
