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Restriction Enzyme Cloning: A Step-by-Step Guide
Restriction–ligation cloning is still the workhorse for moving a fragment into a vector. This guide covers the whole workflow — choosing enzymes, […]
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Vector Builder
Vector Builder is a really useful free online tool where you can design (and purchase) a custom-built vector with a few clicks. […]
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PlasMapper: plasmid drawing
PlasMapper draws an annotated plasmid map from a DNA sequence — automatically detecting and labelling features such as ORFs, restriction sites, promoters, […]
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Cloning: molar ratio of Insert to Vector
Calculate how much insert to add to a ligation for a given insert:vector molar ratio. Enter the vector and insert lengths, the […]