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Codon Table (Standard Genetic Code)
The codon table (standard genetic code) maps each of the 64 three-base codons to an amino acid or a stop signal. Use […]
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Protein Concentration from A280
Estimate protein concentration from an A280 absorbance reading. Enter the A280, your dilution and path length, and the protein’s extinction coefficient (or […]
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GC Content Calculator
Calculate the GC content of a DNA or RNA sequence — the percentage of guanine and cytosine bases. Paste your sequence to […]
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Molarity Calculator
Work out how much of a compound to weigh out for a solution of a given molar concentration and volume. Enter the […]
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Dilution Calculator (C1V1 = C2V2)
Calculate how to dilute a stock solution to a target concentration using C1V1 = C2V2. Enter the stock concentration, the final concentration […]
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DNA and RNA Quantification: Concentration, Purity and Yield
How to measure the concentration, purity and yield of DNA and RNA — the spectrophotometric and fluorometric methods, what the ratios mean, […]
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qPCR Data Analysis: Efficiency, Reference Genes and ΔΔCq
This guide walks through analysing qPCR data: building a standard curve, checking amplification efficiency, choosing reference genes, and quantifying with the ΔΔCq […]
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Restriction Enzyme Cloning: A Step-by-Step Guide
Restriction–ligation cloning is still the workhorse for moving a fragment into a vector. This guide covers the whole workflow — choosing enzymes, […]
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How to Design PCR Primers: A Step-by-Step Guide
Good primers are the difference between a clean PCR band and a smear of non-specific products. This guide walks through the rules […]
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NEBcutter V3.0
NEBcutter V3.0 is New England Biolabs’ tool for finding the restriction enzyme sites in a DNA sequence. Paste your sequence into the […]